Zeiss KF 2 ICS Especificações descarregar pdf (Página 71)

Operation

Axiostar plus Setting epi-fluorescence Carl Zeiss

B 40-815 e 12/01 3-15

The first epi-fluorescence setting is significantly

easier if you use the Plan-Neofluar 20x/0.50

objective and a strong fluorescent specimen.

Demonstration specimens can be used first.

• First leave the illumination beam closed;

move the filter stop carrier 2 (3-10/2) to the

closed position.

• Turn on the HBO 50 with the power supply

and let it warm up to operating

temperature for about 15 minutes.

• Use the adjustment handle (3-10/3) to

move the desired fluorescence filter

combination on the reflector carrier into the

beam path.

• Turn off the halogen lamp (on microscope)

and open illumination beam path. Move the

filter stop carrier 2 to the open position.

• Refocus on the specimen and, if necessary,

optimize the collector setting with the knob

(3-10/1) on the HBO 50. Set the collector so

that the illumination from the field of view

appears as equal as possible to the short-

wave excitation from the reflector module.

Correction of the collector position is no

longer necessary for modules with long-

wave excitation.

1 Knob for collector adjustment

2 Filter stop carrier 2

3 Adjustment handle

Fig. 3-10 Setting epi-fluorescence

1 2 ... 66 67 68 69 70 71 72 73 74 75 76 ... 99 100

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Zeiss KF 2 ICS Especificações Página 71