Zeiss Axioplan Universal microscope Manual do Utilizador descarregar pdf (Página 7)

Human endothelium cells.

Quadruple fluorescence:

DAPI, Alexa 350, Alexa 488,

Phalloidin-Alexa 594.

Plan-Neofluar 63/1.25 oil.

J. Zbaeren, Insel Hospital, Bern

The decisive gain in contrast:

FITC fluorescence micrograph

without Light Trap (1),

with Light Trap (2)

Bright And Brilliant

Perfect in every

dimension:

The new optics

The superbly corrected optics of

Axioplan 2 imaging guarantee

optimal chromatically corrected

illumination in the image plane.

Where light intensities are very

low, an optional 5-element chro-

matic collector further enhances

the illumination homogeneity. Im-

proving color correction, it assures

maximum excitation and, as a

result, maximum emission. High-

performance filters made to very

close tolerances help avoid pixel

shifts. The newly designed reflect-

ed-light beam path allows the sys-

tem to be tailored either for best

transmission or for diffraction-lim-

ited imaging of the field dia-

phragm. Thanks to interchange-

able diaphragm sliders, the user

can select diaphragm formats or

modules.

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