Zeiss LSM 510 META Especificações Página 249

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OPERATION
LSM 510 Annex
B 40-051 e 07/98 4-191
To use the monitordiode for ratio application the following steps have to be done:
Activate the ratio channel R1 or R2 in <Ratio Settings> of the menu <Configuration Control> in
addition to the monitor diode channel (ChM-1) and one PMT channel.
Choose the appropriate PMT channel as source 1 in <Ratio settings> and ChM-1 as source 2. If this
numbering is changed (inverted) the ratio image will show a invertation of gray levels if compared to
the PMT image.
Fig. 4-178
It is not possible to do the ratio between an on-line ratio image generated with two PMT
channels (as in ion-concentration sensitive ratio imaging) and the signal of the monitor diode.
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